Autosampler INTEGRITY™

The new INTEGRITY™ autosampler from Spark sets a new standard in sample care.

  • sample and tray bar code reading
  • ‹ 20 sec injection cycle
  • true 4 °C sample cooling of all samples
  • injection performance monitoring
  • vial bottom detection – inject 1 μL out of 1 μL
  • advanced wash capabilities to eliminate carryover
  • dual independent concentric needle concept
  • optimized for UHPLC
  • advanced reagent addition and derivatization
  • integrated on-line sample prep capabilities
  • up to 4 well plates or 216 standard sample vials
  • flexible workstation for many liquid handling needs of analytical samples

Sample care first

A number of innovative features ensure better and safer control of handling and tracking of samples – ensuring that you feel much more confident about the results.

SBI™ – Zero doubts on sample identity

1D and 2D sample and sample tray identification codes can be read, filed and linked to sample assay results. It is even possible to record an image of the vial during sampling for visual verification of the injection and the sample vial afterwards. Combined with positive feedback motion control for the sample tray positioning, the risk of assigning results to the wrong sample is zero.

IPM™ – Injection performance monitoring 

Injecting the proper sample is one thing but how to verify that the injection itself has been performed correctly? The answer is pressure monitoring of the sample flow path during the entire injection cycle! Pressure profiles of injection cycles are recorded for every injection and by comparison with reference profiles, indications for malfunctioning caused by blockage, leakage, air bubbles, needle damage, valve problems, etc can be easily recognized in case of suspected erroneous results.

INTEGRITY™Instrument controlSafetyCompliancy
Injection concept Pressure Assisted Sample Aspiration (PASA™) using ~ 7 PSI sample headspace pressure (built-in compressor) to avoid air bubbles in sample lines. Unsealed wells or vials can be used without PASA™
Injection volume Programmable from 1 – 10000 μL. Actual volume range depends on
installed sample loop volume
Injection Performance Monitoring
Pressure sensor in sampling flow path generates pressure-time plot for
diagnostic purposes.

  • 0-9999 mbar absolute pressure read out
  • sampling frequency: 10 ms

For OEM purposes: other sampling frequencies are possible, max 30,000 data points

Needle piercing accuracy ± 0.6 mm
Needle wash Freely programmable volumes and speed for wash of sample needle.
2 solvents for inside surface and 2 for outside surface of needle from
external wash solvent bottles.
Note: Option to extend inside and outside wash with 2 extra solvents
Dispenser syringe range 25, 50, 100, 250, 1.000 und 2.500 μL
(user exchangeable; screw lock)
Sample capacity
  • 2 well plates according SBS dimension standards
  • 96 well and 384 well format, low- and high-well (2 plates must be of the same type)
Maximum vial or plate height 48 mm (including septa or cap-mat)
Mix and dilute 4 reagent positions of 450 μL each

  • Standard: 1 reagent pump for replenishment (also used for μL pick-up injections)
  • Option: 3 extra reagent delivery pumps
Valve switching time < 100 msec
Power requirements 100 – 240 Volt AC ±10 %; 50 – 60 Hz
Power consumption 450 VA
Sound pressure level LAeq < 70 dB
Working temperatur 10 – 40 °C
Storage temperatur -25 bis +60 °C

  • Integrity Standard (HPLC/UHPLC/Micro)
  • Integritiy Standard + sample cooling
  • Integrity Plus
  • Integrity Plus with sample cooling
 (Width x Depth x Height)

  • 330 x 540 x 480 mm
  • 330 x 620 x 480 mm
  • 330 x 680 x 480 mm
  • 330 x 760 x 480 mm

  • Integrity Standard (HPLC/UHPLC/Micro)
  • Integrity Standard + Probenkühlung
  • Integrity Plus
  • Integrity Plus mit Probenkühlung

  • 26 kg
  • 30 kg
  • 31 kg
  • 35 kg
Instrument control
PC Interface
  • 2 x Sub-D connector mit RS232 Protokoll and Spark Multilink connection
  • Ethernet RJ45 connector
  • USB
  • programmable relay outputs; NO and NC connectable
  • 1 programmable TTL output; programmable as: Inject marker, Auxiliary
    or Alarm
Inputs 4 programmable TTL inputs for OEM applications
Software SparkLink instrument control software including Integrity driver
Door sensor Upon opening the front door, sample processing will be stopped and the tray will be moved to front after completing the current sample run
Missing vial/well plate sensor Stops run when detecting missing well plate.
User selectable response to missing vial: skip sample or stop run
Illumination LED Full color LED with on/off control. Color selectable
Safety and EMC compatibility According to EC-directives. cCSAus (CSA – UL) approved
Pollution degree 2
Quality ISO 9001
Part number Short description
0950.201 INTEGRITY™ UHPLC Cool
0950.206 INTEGRITY™ Micro UHPLC cool
0950.299 INTEGRITY™ UHPLC full option
sample cooling, multiple wash solvents, barcode reader, HoMix™ and 4 reagent pumps
0951.200 INTEGRITY™ UHPLC Plus
0951.201 INTEGRITY™ UHPLC Plus – Cool
0950.852 extended wash solvent delivery
0950.860 HoMix™ (Homogenizer/mixer modul)
0950.861 SBI (plate/vial barcode scanner)
Important note: HoMix™ module must be installed for reading barcodes on vials
0950.862 ISS 6-port (one additional 6 port switching valve)
Note: max 2 additional switching valves can be installed
0950.863 HotCap™ (heated capillary)
0950.864 extended reagent delivery (3 additional reagent pumps)
0950.865 ISS 10-port (one additional 10 port switching valve)
Note: max 2 additional switching valves can be installed
0950.866 Prep LC (Serum sample needle, Prep vial adaptor, large-bore injection valve, 2.5 mL syringe and 10 ml sample loop)
0950.867 Bio-kompatible HPLC (PEEK injection valve and 100 μL PEEK sample loop, bio-needle)

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